2024
Characterization of LTBP2 mutation causing mitral valve prolapse
Shpitzen S, Rosen H, Ben-Zvi A, Meir K, Levin G, Gudgold A, Dor S, Haffner R, Zwas D, Leibowitz D, Slaugenhaupt S, Banin E, Mizrachi R, Obolensky A, Levine R, Gilon D, Leitersdorf E, Tessler I, Reshef N, Durst R. Characterization of LTBP2 mutation causing mitral valve prolapse. European Heart Journal Open 2024, 5: oeae106. PMID: 39882270, PMCID: PMC11775471, DOI: 10.1093/ehjopen/oeae106.Peer-Reviewed Original ResearchMitral valve prolapseValve prolapseKO miceValve phenotypeMouse strainsTGF-bDisorder associated with significant morbidityReduced visual acuityHuman mutationsOptical coherence tomographyKO mouse strainsLTBP2 mutationsAnterior chamberVisual acuityPolymerase chain reaction analysisSignificant morbidityM mutationMyxomatous changesKnockout miceCoherence tomographyHistological phenotypeKI miceExome sequencingEchocardiographyQuantitative polymerase chain reaction analysisMechanistic Investigation of Human DPP9 Deficiency
Xiao T, Brewer J, Zhou L, Han A, Takabe Y, Carlino M, Blackburn H, Flavell R. Mechanistic Investigation of Human DPP9 Deficiency. Blood 2024, 144: 5699-5699. DOI: 10.1182/blood-2024-211123.Peer-Reviewed Original ResearchHuman HSPCsDevelopment of human immune systemHuman NLRP1Loss of hematopoietic cellsHumanized mouse modelHuman hematopoietic stemStem cells in vivoSensors of infectionLaboratory mouse strainsCells in vivoHuman immune systemMouse genomeHematopoietic stemHuman hematopoiesisHematopoietic cellsCellular stressNegative regulatorPancytopeniaProgenitor cellsMouse strainsRegulatory pathwaysMouse modelHSPCsImmune systemDeletionTert-expressing cells contribute to salivary gland homeostasis and tissue regeneration after radiation therapy
Guan L, Viswanathan V, Jiang Y, Vijayakumar S, Cao H, Zhao J, Colburg D, Neuhöfer P, Zhang Y, Wang J, Xu Y, Laseinde E, Hildebrand R, Rahman M, Frock R, Kong C, Beachy P, Artandi S, Le Q. Tert-expressing cells contribute to salivary gland homeostasis and tissue regeneration after radiation therapy. Genes & Development 2024, 38: 569-582. PMID: 38997156, PMCID: PMC11293384, DOI: 10.1101/gad.351577.124.Peer-Reviewed Original ResearchConceptsSubmandibular glandSalivary gland homeostasisProgenitor cellsGland homeostasisResponse to radiotherapyAdult submandibular glandCell survivalSalivary gland regenerationSelf-renewal capacityEnhanced proliferative activityRadiation therapyDuctal regionsRadiotherapyModulate cell survivalTelomerase-expressingGland regenerationProliferative activityMouse strainsTERT expressionCreERT2 recombinaseSalivary gland biologyRadiation exposureTERT locusIn vitro cultureCell populationsAbstract 5442: Terthighcells: key players in salivary gland homeostasis and regeneration after radiation therapy in adult mice
Guan L, Viswanathan V, V S, Cao H, Jiang Y, Zhao J, Colburg D, Neuhoefer P, Xu Y, Laseinde E, Artandi S, Le Q. Abstract 5442: Terthighcells: key players in salivary gland homeostasis and regeneration after radiation therapy in adult mice. Cancer Research 2024, 84: 5442-5442. DOI: 10.1158/1538-7445.am2024-5442.Peer-Reviewed Original ResearchSalivary gland homeostasisSubmandibular glandRadiation therapyGland homeostasisDuctal regionsAdult miceAmerican Association for Cancer Research annual meetingsProgenitor cellsAcinar cellsRadiation cell killingAdult submandibular glandCell survivalSelf-renewal capacityPost-radiotherapyPost-radiationModulate cell survivalStem/progenitor cellsNormal organsMouse strainsTERT expressionCell killingDuctal cellsOxidative stress response pathwayCreERT2 recombinaseTERT locus
2023
Experimental and phylogenetic evidence for correlated gene expression evolution in endometrial and skin fibroblasts
Dighe A, Maziarz J, Ibrahim-Hashim A, Gatenby R, Kshitiz, Levchenko A, Wagner G. Experimental and phylogenetic evidence for correlated gene expression evolution in endometrial and skin fibroblasts. IScience 2023, 27: 108593. PMID: 38174318, PMCID: PMC10762354, DOI: 10.1016/j.isci.2023.108593.Peer-Reviewed Original ResearchGene expression changesGene expression evolutionEndometrial stromal fibroblastsExpression evolutionExpression changesCell typesGene expressionSimilar gene expression changesSubstantial gene expression changesGene expression profilesSkin fibroblastsMultiple cell typesEvolutionary correlationPhylogenetic evidenceEvolutionary changeDermal skin fibroblastsMammalian speciesExpression profilesPlacental invasivenessComparative datasetCancer growthCultured skin fibroblastsStromal fibroblastsFibroblastsMouse strains
2022
Genotypic Differences in the Effects of Menthol on Nicotine Intake and Preference in Mice
Akinola LS, Rahman Y, Ondo O, Gonzales J, Bagdas D, Jackson A, Davidson-Wert N, Damaj MI. Genotypic Differences in the Effects of Menthol on Nicotine Intake and Preference in Mice. Frontiers In Neuroscience 2022, 16: 905330. PMID: 35769694, PMCID: PMC9234577, DOI: 10.3389/fnins.2022.905330.Peer-Reviewed Original ResearchOral nicotine consumptionEffect of mentholD2J miceMenthol effectsNicotine consumptionΑ7 nAChRsDifferential effectsTwo-bottle choiceTAAR1 geneOral aversionC57BL/6J miceNicotine rewardSystemic administrationNicotine intakeDBA/2J miceB6J micePharmacological responseNicotine addictionGenotype-specific mechanismsNicotine concentrationsMiceMouse strainsBasal preferenceGenetic factorsNicotine
2021
Macroscopic Structural and Connectome Mapping of the Mouse Brain Using Diffusion Magnetic Resonance Imaging
Arefin TM, Lee CH, White JD, Zhang J, Kaffman A. Macroscopic Structural and Connectome Mapping of the Mouse Brain Using Diffusion Magnetic Resonance Imaging. Bio-protocol 2021, 11: e4221. PMID: 34909442, PMCID: PMC8635841, DOI: 10.21769/bioprotoc.4221.Peer-Reviewed Original ResearchEarly life stressMagnetic resonance imagingMouse brainDiffusion magnetic resonance imagingMouse modelResonance imagingWhole-brain voxel-based analysisVoxel-based analysisClinical trialsPostnatal stressPreclinical studiesNeurological conditionsAdult miceEntire mouse brainStructural abnormalitiesBrain regionsMouse strainsBrain connectivityBrainLife stressTranslational utilityStructural connectivityRodentsMiceTranslational workSex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure
Lee AM, Mansuri MS, Wilson RS, Lam TT, Nairn AC, Picciotto MR. Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure. Frontiers In Molecular Neuroscience 2021, 14: 657064. PMID: 34335180, PMCID: PMC8317211, DOI: 10.3389/fnmol.2021.657064.Peer-Reviewed Original ResearchVentral tegmental areaC3H/HeJ miceGlial fibrillary acidic proteinChronic nicotine administrationNicotine administrationProtein abundanceProteomeIsobaric labelingNicotine exposureFemale miceTegmental areaHeJ miceNucleus accumbensNicotine addictionProteinSex differencesSample fractionationPathwayFibrillary acidic proteinTandem mass spectrometryNetwork analysisMouse strainsChronic nicotineMesolimbic systemNicotine rewardEndothelial SIRT3 regulates myofibroblast metabolic shifts in diabetic kidneys
Srivastava SP, Li J, Takagaki Y, Kitada M, Goodwin JE, Kanasaki K, Koya D. Endothelial SIRT3 regulates myofibroblast metabolic shifts in diabetic kidneys. IScience 2021, 24: 102390. PMID: 33981977, PMCID: PMC8086030, DOI: 10.1016/j.isci.2021.102390.Peer-Reviewed Original ResearchDiabetic kidney fibrosisDiabetic kidneyEndothelial cellsKidney fibrosisDefective metabolismRenal tubular epithelial cellsTubular epithelial cellsKidney functionDiabetic miceFibrogenic pathwaysFibrogenic processDisease processLoss of functionMesenchymal transitionKidneyMouse strainsEpithelial cellsGain of functionSIRT3Metabolic reprogrammingMesenchymal transformationFibrosisSIRT3 geneMetabolismCells
2020
PLD3 is a neuronal lysosomal phospholipase D associated with β‐amyloid plaques and cognitive function in Alzheimer’s disease
Nackenoff A, Hohman T, Neuner S, Akers C, Weitzel N, Shostak A, Ferguson S, Bennett D, Schneider J, Jefferson A, Kaczorowski C, Schrag M. PLD3 is a neuronal lysosomal phospholipase D associated with β‐amyloid plaques and cognitive function in Alzheimer’s disease. Alzheimer's & Dementia 2020, 16 DOI: 10.1002/alz.043301.Peer-Reviewed Original ResearchSporadic Alzheimer's diseaseΒ-amyloid plaquesAlzheimer's diseaseCerebral β-amyloidosisΒ-amyloid pathologyPhospholipase D3Normal human brainPre-frontal cortexAD-affected brainsFear conditioning taskReligious Orders StudyDystrophic neuritesAD brainΒ-amyloidosisMouse modelCognitive declineMouse brainPhospholipase D isoformsCognitive functionPathology severityMouse strainsDiseaseBrainRush MemoryMRNA levelsGenetic determinants of ammonia-induced acute lung injury in mice
Bein K, Ganguly K, Martin TM, Concel VJ, Brant KA, Di YPP, Upadhyay S, Fabisiak JP, Vuga LJ, Kaminski N, Kostem E, Eskin E, Prows DR, Jang AS, Leikauf GD. Genetic determinants of ammonia-induced acute lung injury in mice. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2020, 320: l41-l62. PMID: 33050709, PMCID: PMC7847062, DOI: 10.1152/ajplung.00276.2020.Peer-Reviewed Original ResearchConceptsSNP associationsWide association mappingGenetic determinantsSignificant SNP associationsAcute lung injuryIntegrative functional approachAssociation mappingMolecular functionsTranscriptomic analysisCandidate genesFunctional domainsNonsynonymous SNPsPromoter regionLung injuryDiverse panelGenesSNPsMouse strainsPathophysiological roleAATFInjuryProteinLAMA3ExpressionAssemblyResolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter
Eastman AE, Chen X, Hu X, Hartman AA, Morales A, Yang C, Lu J, Kueh HY, Guo S. Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter. Cell Reports 2020, 31: 107804. PMID: 32579930, PMCID: PMC7418154, DOI: 10.1016/j.celrep.2020.107804.Peer-Reviewed Original ResearchConceptsFluorescent reportersLive-cell fluorescent reporterCell cycle speedFluorescent timer proteinsCell proliferationCell cycle dynamicsRed fluorescent proteinFaster cycling cellsFate transitionsFusion reporterActive lociTimer proteinFluorescent proteinLength heterogeneityComplex tissuesHematopoietic cellsCycling cellsReporterFluorescence ratioCycle dynamicsProteinFunctional heterogeneityMouse strainsSolid tissuesCycle speedAn Immunologic Mode of Multigenerational Transmission Governs a Gut Treg Setpoint
Ramanan D, Sefik E, Galván-Peña S, Wu M, Yang L, Yang Z, Kostic A, Golovkina T, Kasper D, Mathis D, Benoist C. An Immunologic Mode of Multigenerational Transmission Governs a Gut Treg Setpoint. Cell 2020, 181: 1276-1290.e13. PMID: 32402238, PMCID: PMC7393667, DOI: 10.1016/j.cell.2020.04.030.Peer-Reviewed Original ResearchConceptsDouble-negative feedback loopTreg proportionImmunological modeGut immune responseGut commensalsControlling gut inflammationSpecies levelInbred mouse strainsMulti-generational transmissionTreg-dependent mannerCellular perturbationsRegulatory T cellsDisease susceptibilityNon-epigeneticMaternal transmissionInflammatory disease susceptibilityNon-geneticGut inflammationT cellsGenetic tuningMouse strainsImmune responseMultiple generationsImmune systemFeedback loop
2019
Cellular Differences in the Cochlea of CBA and B6 Mice May Underlie Their Difference in Susceptibility to Hearing Loss
Liu H, Li G, Lu J, Gao Y, Song L, Li G, Wu H. Cellular Differences in the Cochlea of CBA and B6 Mice May Underlie Their Difference in Susceptibility to Hearing Loss. Frontiers In Cellular Neuroscience 2019, 13: 60. PMID: 30873008, PMCID: PMC6400987, DOI: 10.3389/fncel.2019.00060.Peer-Reviewed Original ResearchInner hair cellsEarly-onset hearing lossB6 miceOnset hearing lossHearing lossRibbon synapsesOuter hair cell functionMouse strainsCBA/CaJ miceWave I latencySpiral ganglion neuronsSynaptic vesiclesHair cell functionCellular differencesStable hearingSimilar hearing thresholdsI latencyABR thresholdGanglion neuronsGlutamate toxicityHearing thresholdsWave IMiceHair cellsCell function
2018
A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus
Uchil PD, Pi R, Haugh KA, Ladinsky MS, Ventura JD, Barrett BS, Santiago ML, Bjorkman PJ, Kassiotis G, Sewald X, Mothes W. A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus. Cell Host & Microbe 2018, 25: 87-100.e10. PMID: 30595553, PMCID: PMC6331384, DOI: 10.1016/j.chom.2018.11.011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell ProliferationDendritic CellsDisease Models, AnimalErythroblastsFemaleInterferon Type ILectinsLymph NodesMacrophagesMaleMiceMice, Inbred BALB CMice, Inbred C57BLProtective AgentsRetroviridaeRetroviridae InfectionsSialic Acid Binding Ig-like Lectin 1SpleenT-Lymphocytes, CytotoxicViral LoadConceptsCD169/SiglecEffective cytotoxic T lymphocyte (CTL) responseProtective roleCytotoxic T lymphocyte responsesLymph node infectionT lymphocyte responsesHigh viral loadSusceptible mouse strainsMarginal zone metallophilic macrophagesPermissive lymphocytesCytotoxic CD8Lymphocyte responsesViral loadSubcapsular sinusComplex infectionMurine modelViral disseminationMetallophilic macrophagesRed pulpCell responsesSystemic spreadMouse strainsPathogenesisCells 1CD169Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development
Konjikusic MJ, Yeetong P, Boswell CW, Lee C, Roberson EC, Ittiwut R, Suphapeetiporn K, Ciruna B, Gurnett CA, Wallingford JB, Shotelersuk V, Gray RS. Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development. PLOS Genetics 2018, 14: e1007817. PMID: 30475797, PMCID: PMC6307780, DOI: 10.1371/journal.pgen.1007817.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnimals, Genetically ModifiedBase SequenceChildCiliaConsanguinityEpendymaFemaleGene ExpressionHomozygoteHumansHydrocephalusIntellectual DisabilityKinesinsMaleMiceMice, TransgenicModels, AnimalMutationNeurodevelopmental DisordersPedigreeSequence DeletionTissue DistributionXenopus laevisZebrafishConceptsVentricular systemMulti-ciliated cellsNeurological developmentEpendymal cellsHuman neurological developmentKinesin family member 6C-terminal truncating mutationsMember 6 geneEpendymal cell ciliaTransgenic mouse strainCerebrospinal fluid flowMutant mice displayFamily member 6Homozygous null mutationMice displaySpecific roleMutant miceMouse strainsNeurodevelopmental defectsTruncating mutationsMember 6Multiciliated tissuesIntellectual disabilityBase pair deletionMiceCharacterizing a novel vGlut3-P2A-iCreER knockin mouse strain in cochlea
Li C, Shu Y, Wang G, Zhang H, Lu Y, Li X, Li G, Song L, Liu Z. Characterizing a novel vGlut3-P2A-iCreER knockin mouse strain in cochlea. Hearing Research 2018, 364: 12-24. PMID: 29706463, DOI: 10.1016/j.heares.2018.04.006.Peer-Reviewed Original ResearchMeSH KeywordsAcoustic StimulationAmino Acid Transport Systems, AcidicAnimalsCochleaEvoked Potentials, Auditory, Brain StemFemaleGene Knock-In TechniquesGenes, ReporterGenotypeHair Cells, Auditory, OuterIntegrasesLuminescent ProteinsMaleMice, Inbred C57BLMice, TransgenicNeurogliaPhenotypeReaction TimeReceptors, EstrogenSelective Estrogen Receptor ModulatorsSpiral GanglionTamoxifenTime FactorsConceptsInner hair cellsOuter hair cellsSpiral ganglion neuronsKnockin mouse strainGlia cellsVesicular glutamate transporter 3Mouse strainsHair cellsCochlear outer hair cellsRosa26-LSLVGLUT3 expressionGanglion neuronsVGLUT3Mouse cochleaTransporter 3Negative cellsMouse genetic studiesAntibody stainingTamoxifenUnique expression patternP2/P3Specific cell typesCell typesSitu hybridizationCochleaMast Cells Are Activated by Streptococcus pneumoniae In Vitro but Dispensable for the Host Defense Against Pneumococcal Central Nervous System Infection In Vivo
Fritscher J, Amberger D, Dyckhoff S, Bewersdorf J, Masouris I, Voelk S, Hammerschmidt S, Schmetzer H, Klein M, Pfister H, Koedel U. Mast Cells Are Activated by Streptococcus pneumoniae In Vitro but Dispensable for the Host Defense Against Pneumococcal Central Nervous System Infection In Vivo. Frontiers In Immunology 2018, 9: 550. PMID: 29616039, PMCID: PMC5867309, DOI: 10.3389/fimmu.2018.00550.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsCell DegranulationCells, CulturedCentral Nervous SystemCromolyn SodiumHumansImmunity, InnateMaleMast CellsMeningitis, PneumococcalMiceMice, Inbred C57BLMice, TransgenicMutationPneumococcal InfectionsProto-Oncogene Proteins c-kitStreptococcus pneumoniaeStreptolysinsConceptsBone marrow-derived mast cellsCentral nervous systemSystemic infection in vivoMast cellsBone-marrow-derived mast cell degranulationMast cell engraftmentMouse bone marrow-derived mast cellsBacterial infectionsMarrow-derived mast cellsCerebrospinal fluidMutant mouse strainsMast cell-deficientExperimental pneumococcal meningitisMast cell stabilizerSystemic bacterial infectionInfection in vivoDisease phenotypeCell deficiencyCSF pleocytosisPneumococcal serotypesC-kitCell engraftmentPneumococcal meningitisMouse strainsNervous systemA Role for the Non-Receptor Tyrosine Kinase Abl2/Arg in Experimental Neuroinflammation
Jacobsen FA, Scherer AN, Mouritsen J, Bragadóttir H, Thomas Bäckström B, Sardar S, Holmberg D, Koleske AJ, Andersson Å. A Role for the Non-Receptor Tyrosine Kinase Abl2/Arg in Experimental Neuroinflammation. Journal Of Neuroimmune Pharmacology 2018, 13: 265-276. PMID: 29550892, PMCID: PMC5928183, DOI: 10.1007/s11481-018-9783-8.Peer-Reviewed Original ResearchConceptsMultiple sclerosisExperimental autoimmune encephalomyelitis (EAE) modelT cell receptorCongenic mouse strainsExperimental neuroinflammationInflammation pathogenesisMyelin antigensEAE developmentExperimental arthritisAutoimmune responseImmune cellsDisease susceptibility factorsCongenic miceLymphocyte activationPharmacological inhibitionAbl kinaseSclerosisSusceptibility factorsMouse strainsDegenerative diseasesPotential drug targetsMouse genetic locusSingle nucleotide polymorphismsDisease etiologyAmino acid changes
2016
Outbred CD1 mice are as suitable as inbred C57BL/6J mice in performing social tasks
Hsieh LS, Wen JH, Miyares L, Lombroso PJ, Bordey A. Outbred CD1 mice are as suitable as inbred C57BL/6J mice in performing social tasks. Neuroscience Letters 2016, 637: 142-147. PMID: 27871995, PMCID: PMC5203811, DOI: 10.1016/j.neulet.2016.11.035.Peer-Reviewed Original ResearchConceptsOutbred CD1 miceC57 miceCD1 miceAge-matched male miceThree-chamber sociability testThree-chamber testThree-chamber taskStranger mouseSocial interaction testAggressive behaviorMale CD1C57BL/6J miceMale miceBehavioral testingSociability testOutbred miceMiceMouse strainsStatistical significanceInteraction testMore time
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