2022
Structural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide
Zhao Y, Roy K, Vidossich P, Cancedda L, De Vivo M, Forbush B, Cao E. Structural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide. Nature Communications 2022, 13: 2747. PMID: 35585053, PMCID: PMC9117670, DOI: 10.1038/s41467-022-30407-3.Peer-Reviewed Original ResearchConceptsTranslocation pathwayElectron cryo-microscopy structureC-terminal domainIon translocation pathwayCation-chloride cotransporters NKCC1Transmembrane domainCotransporter NKCC1C-terminal domain interactionsStructural basisDomain interactionsRenal salt reabsorptionDomain associationConformational changesFunctional studiesIon translocationElectroneutral symportCell membraneNKCC1PathwayNKCC2DomainSalt reabsorptionTransmembraneTranslocationTransporters
2020
Cannabidiol interactions with voltage-gated sodium channels
Sait LG, Sula A, Ghovanloo MR, Hollingworth D, Ruben PC, Wallace B. Cannabidiol interactions with voltage-gated sodium channels. ELife 2020, 9: e58593. PMID: 33089780, PMCID: PMC7641581, DOI: 10.7554/elife.58593.Peer-Reviewed Original ResearchConceptsVoltage-gated sodium channelsNavMs voltage-gated sodium channelHigh-resolution X-ray crystallographyIon translocation pathwaySodium channelsCentral hydrophobic cavitySodium channel mutationsTranslocation pathwayMolecular mechanismsNovel siteNovel insightsTRPV2 channelsTarget siteChannel mutationsX-ray crystallographyNon-psychoactive compoundFunctional effectsHydrophobic cavityCannabis plantChannel inhibitionSitesPlantsMutationsNeurological diseasesType of epilepsy
2013
Spectral Tuning in Halorhodopsin: The Chloride Pump Photoreceptor
Pal R, Sekharan S, Batista V. Spectral Tuning in Halorhodopsin: The Chloride Pump Photoreceptor. Journal Of The American Chemical Society 2013, 135: 9624-9627. PMID: 23777372, DOI: 10.1021/ja404600z.Peer-Reviewed Original ResearchConceptsKey amino acid residuesIon translocation pathwayAmino acid residuesSpectral tuningInternal water moleculesTranslocation pathwaySpecific hydrogen bondsHalobacterium salinarumAcid residuesMolecular levelStructural rearrangementsAnion transportCl depletionPhotoreceptorsInduces changesHalorhodopsinRetinyl chromophoreSalinarumMutationsResiduesPathwayFirst time
2012
Loop Diuretic and Ion-binding Residues Revealed by Scanning Mutagenesis of Transmembrane Helix 3 (TM3) of Na-K-Cl Cotransporter (NKCC1)*
Somasekharan S, Tanis J, Forbush B. Loop Diuretic and Ion-binding Residues Revealed by Scanning Mutagenesis of Transmembrane Helix 3 (TM3) of Na-K-Cl Cotransporter (NKCC1)*. Journal Of Biological Chemistry 2012, 287: 17308-17317. PMID: 22437837, PMCID: PMC3366785, DOI: 10.1074/jbc.m112.356014.Peer-Reviewed Original ResearchConceptsTransmembrane helix 3Na-K-Cl cotransporterTranslocation pathwayHelix 3Homology modelTryptophan-scanning mutagenesisMutation of residuesStructural homology modelEpithelial salt transportExtracellular gateCellular chloride homeostasisScanning mutagenesisOpen conformationIntracellular endPore residuesFunctional roleIon translocationTranslocation rateResiduesMutagenesisCentral roleChloride homeostasisMutationsPathwayLarge effect
2006
In Vivo Membrane Topology of Escherichia coli SecA ATPase Reveals Extensive Periplasmic Exposure of Multiple Functionally Important Domains Clustering on One Face of SecA*
Jilaveanu LB, Oliver DB. In Vivo Membrane Topology of Escherichia coli SecA ATPase Reveals Extensive Periplasmic Exposure of Multiple Functionally Important Domains Clustering on One Face of SecA*. Journal Of Biological Chemistry 2006, 282: 4661-4668. PMID: 17166834, DOI: 10.1074/jbc.m610828200.Peer-Reviewed Original ResearchConceptsSecA ATPaseEscherichia coli SecA ATPaseProtein translocation pathwayProtein-conducting channelRegion of SecABacterial plasma membraneMembrane-integrated stateTransport of proteinsSecA mutantSecA domainProtein translocationMembrane topologyMembrane cyclingSecA structureMembrane insertionTranslocation pathwayProtein cargoPlasma membraneSulfhydryl labelingN-domainSecAC-domainTrans sideChannel complexLabeling patterns
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