2024
Periodic ER-plasma membrane junctions support long-range Ca2+ signal integration in dendrites
Benedetti L, Fan R, Weigel A, Moore A, Houlihan P, Kittisopikul M, Park G, Petruncio A, Hubbard P, Pang S, Xu C, Hess H, Saalfeld S, Rangaraju V, Clapham D, De Camilli P, Ryan T, Lippincott-Schwartz J. Periodic ER-plasma membrane junctions support long-range Ca2+ signal integration in dendrites. Cell 2024, 188: 484-500.e22. PMID: 39708809, DOI: 10.1016/j.cell.2024.11.029.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium SignalingCalcium-Calmodulin-Dependent Protein Kinase Type 2Cell MembraneDendritesEndoplasmic ReticulumHippocampusHumansMaleMiceRatsRyanodine Receptor Calcium Release ChannelConceptsEndoplasmic reticulum-plasma membrane junctionsEndoplasmic reticulum-plasma membranePlasma membrane of dendritesVoltage-gated Ca<sup>2+</sup> channelsER-plasma membrane junctionsMembrane of dendritesProtein kinase IIRyanodine receptorSynaptic inputsDendritic computationsSpine stimulationNeuronal dendritesKinase IIIntracellular signalingMembrane junctionsPlasma membraneER tubulesSignal propagationSignal transmissionSubcellular architectureRyanodineLadder-like arraysLocal activationReleaseDendrites
2022
Impaired Dynamic Sarcoplasmic Reticulum Ca Buffering in Autosomal Dominant CPVT2
Wleklinski M, Kryshtal D, Kim K, Parikh S, Blackwell D, Marty I, Iyer V, Knollmann B. Impaired Dynamic Sarcoplasmic Reticulum Ca Buffering in Autosomal Dominant CPVT2. Circulation Research 2022, 131: 673-686. PMID: 36102198, PMCID: PMC9529867, DOI: 10.1161/circresaha.121.320661.Peer-Reviewed Original ResearchAnimalsCalciumCalcium-Binding ProteinsCalsequestrinCatecholaminesMiceMyocytes, CardiacPolymersRyanodine Receptor Calcium Release ChannelSarcoplasmic ReticulumTachycardia, VentricularClinical and Functional Characterization of Ryanodine Receptor 2 Variants Implicated in Calcium-Release Deficiency Syndrome
Roston TM, Wei J, Guo W, Li Y, Zhong X, Wang R, Estillore JP, Peltenburg PJ, Noguer FRI, Till J, Eckhardt LL, Orland KM, Hamilton R, LaPage MJ, Krahn AD, Tadros R, Vinocur JM, Kallas D, Franciosi S, Roberts JD, Wilde AAM, Jensen HK, Sanatani S, Chen SRW. Clinical and Functional Characterization of Ryanodine Receptor 2 Variants Implicated in Calcium-Release Deficiency Syndrome. JAMA Cardiology 2022, 7: 84-92. PMID: 34730774, PMCID: PMC8567190, DOI: 10.1001/jamacardio.2021.4458.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultChildDeath, Sudden, CardiacElectrocardiographyFemaleFollow-Up StudiesGlobal HealthHumansMaleMorbidityMutationPhenotypeProspective StudiesRetrospective StudiesRyanodine Receptor Calcium Release ChannelTachycardia, VentricularYoung AdultConceptsCatecholaminergic polymorphic ventricular tachycardiaExercise stress testingDeficiency syndromeRYR2 variantsVentricular tachyarrhythmiasArrhythmic eventsVentricular fibrillationLife-threatening arrhythmic eventsMulticenter observational cohort studyFunction variantsCardiac ryanodine receptor 2Complex ventricular tachyarrhythmiasPrevious arrhythmic eventsΒ-blocker therapyObservational cohort studyPolymorphic ventricular tachycardiaLife-threatening eventsSpectrum of diseaseVentricular fibrillation episodesFirst clinical seriesRyanodine receptor 2Better diagnostic toolsCohort studyVentricular arrhythmiasClinical series
2021
Age‐related calcium dysregulation linked with tau pathology and impaired cognition in non‐human primates
Datta D, Leslie SN, Wang M, Morozov YM, Yang S, Mentone S, Zeiss C, Duque A, Rakic P, Horvath TL, van Dyck C, Nairn AC, Arnsten AFT. Age‐related calcium dysregulation linked with tau pathology and impaired cognition in non‐human primates. Alzheimer's & Dementia 2021, 17: 920-932. PMID: 33829643, PMCID: PMC8195842, DOI: 10.1002/alz.12325.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsCalciumCalcium SignalingCognitive DysfunctionCyclic AMP-Dependent Protein KinasesDisease Models, AnimalHumansMacaca mulattaMaleNeuronsPhosphorylationPrefrontal CortexRatsRyanodine Receptor Calcium Release Channeltau ProteinsConceptsTau pathologyCalcium leakTau phosphorylationNeuronal firingAlzheimer's diseaseEarly tau phosphorylationPyramidal cell dendritesSporadic Alzheimer's diseasePrimary cortical neuronsPotential therapeutic targetCognitive performanceAge-related reductionMacaque dorsolateral prefrontal cortexDorsolateral prefrontal cortexNon-human primatesCalcium dysregulationCell dendritesCortical neuronsCalcium-binding proteinsAD biomarkersPathology markersTherapeutic targetAnimal modelsAged monkeysPrefrontal cortex
2017
Thyroid and Glucocorticoid Hormones Promote Functional T-Tubule Development in Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes
Parikh SS, Blackwell DJ, Gomez-Hurtado N, Frisk M, Wang L, Kim K, Dahl CP, Fiane A, Tønnessen T, Kryshtal DO, Louch WE, Knollmann BC. Thyroid and Glucocorticoid Hormones Promote Functional T-Tubule Development in Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes. Circulation Research 2017, 121: 1323-1330. PMID: 28974554, PMCID: PMC5722667, DOI: 10.1161/circresaha.117.311920.Peer-Reviewed Original ResearchMeSH KeywordsCalcium SignalingCell DifferentiationCells, CulturedExcitation Contraction CouplingGlucocorticoidsHumansInduced Pluripotent Stem CellsMyocytes, CardiacRyanodine Receptor Calcium Release ChannelThyroid HormonesConceptsHuman-induced pluripotent stem cell-derived cardiomyocytesExcitation-contraction couplingPluripotent stem cell-derived cardiomyocytesCa releaseGlucocorticoid hormonesStem cell-derived cardiomyocytesCell-derived cardiomyocytesCa-induced Ca releaseT-tubule developmentAdult human ventricular cardiomyocytesExtensive T-tubule networkHuman ventricular cardiomyocytesL-type Ca channelsL-type CaT-tubulesIntracellular Ca releaseCell-based therapiesConfocal line scanHeart diseaseT-tubule networkFunctional maturationVentricular cardiomyocytesPermissive roleDay 16Ca channelsThe clinical and genetic spectrum of catecholaminergic polymorphic ventricular tachycardia: findings from an international multicentre registry
Roston TM, Yuchi Z, Kannankeril PJ, Hathaway J, Vinocur JM, Etheridge SP, Potts JE, Maginot KR, Salerno JC, Cohen MI, Hamilton RM, Pflaumer A, Mohammed S, Kimlicka L, Kanter RJ, LaPage MJ, Collins KK, Gebauer RA, Temple JD, Batra AS, Erickson C, Miszczak-Knecht M, Kubuš P, Bar-Cohen Y, Kantoch M, Thomas VC, Hessling G, Anderson C, Young ML, Choi SHJ, Cabrera Ortega M, Lau YR, Johnsrude CL, Fournier A, Van Petegem F, Sanatani S. The clinical and genetic spectrum of catecholaminergic polymorphic ventricular tachycardia: findings from an international multicentre registry. EP Europace 2017, 20: 541-547. PMID: 28158428, PMCID: PMC6059141, DOI: 10.1093/europace/euw389.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentCalsequestrinChildDeath, Sudden, CardiacDNA Mutational AnalysisFemaleGenetic MarkersGenetic Predisposition to DiseaseHeredityHumansMaleModels, MolecularMutationPedigreePhenotypePrognosisProtein ConformationRegistriesRetrospective StudiesRisk FactorsRyanodine Receptor Calcium Release ChannelStructure-Activity RelationshipTachycardia, VentricularConceptsCatecholaminergic polymorphic ventricular tachycardiaPolymorphic ventricular tachycardiaCardiac eventsCPVT patientsVentricular tachycardiaLife-threatening cardiac eventsInternational multicentre registrySevere CPVT phenotypeRetrospective cohort studyFirst-degree relativesYears of ageRyanodine receptor 2CPVT phenotypeMulticentre registryCohort studySymptomatic patientsMulticentre studyVentricular arrhythmiasCardiac arrestPrognostic markerReceptor 2Ion channelopathiesClinical phenotypeGenetic spectrumGenotypic spectrumSheet-Like Remodeling of the Transverse Tubular System in Human Heart Failure Impairs Excitation-Contraction Coupling and Functional Recovery by Mechanical Unloading
Seidel T, Navankasattusas S, Ahmad A, Diakos N, Xu W, Tristani-Firouzi M, Bonios M, Taleb I, Li D, Selzman C, Drakos S, Sachse F. Sheet-Like Remodeling of the Transverse Tubular System in Human Heart Failure Impairs Excitation-Contraction Coupling and Functional Recovery by Mechanical Unloading. Circulation 2017, 135: 1632-1645. PMID: 28073805, PMCID: PMC5404964, DOI: 10.1161/circulationaha.116.024470.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBiopsyCase-Control StudiesEchocardiographyExcitation Contraction CouplingFemaleHeart FailureHeart-Assist DevicesHumansImage Processing, Computer-AssistedMaleMicroscopy, ConfocalMiddle AgedMyocardial ContractionMyocytes, CardiacProspective StudiesProsthesis DesignRecovery of FunctionRyanodine Receptor Calcium Release ChannelTime FactorsTreatment OutcomeVentricular Function, LeftVentricular RemodelingConceptsLeft ventricular assist deviceChronic heart failureHeart failureExcitation-contraction couplingCardiac recoveryMechanical unloadingLVAD implantationEjection fractionFunctional recoveryHF myocytesImplantation of LVADHallmark of HFHuman end-stage heart failureLeft ventricular ejection fractionEnd-stage heart failureTransverse tubular systemEnd-stage human heart failureEjection fraction increaseIsolated human cardiomyocytesVentricular ejection fractionSubgroup of patientsVolume/length ratioHuman heart failureFunctional cardiac recoveryVentricular assist device
2016
Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3
Linsley JW, Hsu IU, Groom L, Yarotskyy V, Lavorato M, Horstick EJ, Linsley D, Wang W, Franzini-Armstrong C, Dirksen RT, Kuwada JY. Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 114: e228-e236. PMID: 28003463, PMCID: PMC5240691, DOI: 10.1073/pnas.1619238114.Peer-Reviewed Original ResearchConceptsEC couplingDihydropyridine receptorNative American myopathySarcoplasmic reticulumExcitation-contraction couplingSkeletal muscle contractionVoltage-sensing dihydropyridine receptorCaffeine-induced CaRyanodine receptor 1Receptor 1Muscle contractionInternal storesMuscle Ca2Muscle fibersSTAC3Luminal CaMyopathyTransverse tubule membranesTubule membranesCaffeine sensitivityHuman myopathiesCoupling apparatusHuman diseasesDynamic imagingCritical role
2010
Low Extracellular pH Induces Damage in the Pancreatic Acinar Cell by Enhancing Calcium Signaling*
Reed AM, Husain SZ, Thrower E, Alexandre M, Shah A, Gorelick FS, Nathanson MH. Low Extracellular pH Induces Damage in the Pancreatic Acinar Cell by Enhancing Calcium Signaling*. Journal Of Biological Chemistry 2010, 286: 1919-1926. PMID: 21084290, PMCID: PMC3023488, DOI: 10.1074/jbc.m110.158329.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium Channel BlockersCalcium SignalingCells, CulturedHydrogen-Ion ConcentrationPancreas, ExocrinePancreatitisRatsRats, Sprague-DawleyRyanodine Receptor Calcium Release ChannelConceptsPathogenesis of pancreatitisAcinar cellsRyR inhibitorsLow pHeDevelopment of pancreatitisRyanodine receptor inhibitorPancreatic acinar cellsReceptor inhibitorsClinical conditionsCellular injuryPancreatitisBasolateral regionExocrine pancreasPancreatitis responsesInjurious effectsCalcium signalingPathogenesisInduces damageInhibitorsCellsRyRsInjuryEarly stepsPancreasSensitizationDantrolene mitigates caerulein-induced pancreatitis in vivo in mice
Orabi AI, Shah AU, Ahmad MU, Choo-Wing R, Parness J, Jain D, Bhandari V, Husain SZ. Dantrolene mitigates caerulein-induced pancreatitis in vivo in mice. AJP Gastrointestinal And Liver Physiology 2010, 299: g196-g204. PMID: 20448143, PMCID: PMC2904115, DOI: 10.1152/ajpgi.00498.2009.Peer-Reviewed Original ResearchMeSH KeywordsAmylasesAnimalsApoptosisCalcium Channel BlockersCalcium SignalingCeruletideCytoprotectionDantroleneDisease Models, AnimalEnzyme ActivationFluorescent Antibody TechniqueIn Situ Nick-End LabelingMaleMiceMice, Inbred C57BLMicroscopy, ElectronPancreasPancreatitisRyanodine Receptor Calcium Release ChannelSeverity of Illness IndexTime FactorsTrypsinConceptsPancreatic trypsin activityCaerulein-induced pancreatitisRyanodine receptorAcinar cellsMouse pancreatic sectionsHigh-risk settingsTUNEL-positive cellsLater time pointsRyR inhibitor dantroleneDantrolene pretreatmentHistological severityAcute pancreatitisPancreatic acinar cellsInflammatory disordersProphylactic treatmentRyR inhibitionSerum amylasePancreatitis inductionPancreatic sectionsPancreatitisAdequate treatmentTrypsin activityEarly markerCaerulein hyperstimulationUltrastructural derangements
2009
CLIC2-RyR1 Interaction and Structural Characterization by Cryo-electron Microscopy
Meng X, Wang G, Viero C, Wang Q, Mi W, Su XD, Wagenknecht T, Williams AJ, Liu Z, Yin CC. CLIC2-RyR1 Interaction and Structural Characterization by Cryo-electron Microscopy. Journal Of Molecular Biology 2009, 387: 320-334. PMID: 19356589, PMCID: PMC2667806, DOI: 10.1016/j.jmb.2009.01.059.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumChloride ChannelsCryoelectron MicroscopyCrystallography, X-RayIon Channel GatingModels, MolecularMuscle, SkeletalProtein BindingProtein ConformationRabbitsRyanodine Receptor Calcium Release ChannelSarcoplasmic ReticulumSurface PropertiesTritiumConceptsCryo-electron microscopyChannel activitySkeletal ryanodine receptorsAffinity of ryanodineSkeletal sarcoplasmic reticulum vesiclesSmall proteinsClamp regionConformational changesPhysiological functionsDomain 5Closed stateSingle-channel recordingsStructural familyRyR1 channelsRyanodine receptorSkeletal muscleRyR1VesiclesOpen probabilityRyR channelsChannel 2Channel recordingsEfflux rateSarcoplasmic reticulum vesiclesReticulum vesicles
2007
The Perfect Storm
Akar FG. The Perfect Storm. Circulation Research 2007, 101: 968-970. PMID: 17991890, DOI: 10.1161/circresaha.107.164426.Commentaries, Editorials and LettersAction PotentialsAnimalsCalciumMyocytes, CardiacRyanodine Receptor Calcium Release ChannelTachycardia, Ventricular
2006
A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia
Kachoei BA, Knox RJ, Uthuza D, Levy S, Kaczmarek LK, Magoski NS. A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia. Journal Of Neurophysiology 2006, 96: 2688-2698. PMID: 16885525, PMCID: PMC2894935, DOI: 10.1152/jn.00118.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnilidesAnimalsAplysiaCalcium Channel BlockersCalcium ChannelsCalcium SignalingElectrophysiologyEnzyme InhibitorsImidazolesIn Vitro TechniquesIndicators and ReagentsIndolesInositol 1,4,5-TrisphosphateLanthanumMacrocyclic CompoundsMembrane PotentialsNeuronsNickelOxazolesRyanodine Receptor Calcium Release ChannelThapsigarginThiadiazolesConceptsBag cell neuronsCell neuronsCyclopiazonic acidCPA-sensitive storesSmooth muscle cell lineAbsence of extracellularCultured bag cell neuronsRyanodine receptor agonistATPase inhibitorReceptor blockersReceptor agonistNeuropeptide secretionXestospongin CMuscle cell lineInflux pathwayIntracellular storesNonneuronal cellsAcidic storesElevated intracellularNeuronsStore depletionAfterdischargesCell linesBafilomycin AEntry pathwayGenetic ablation of caveolin-1 modifies Ca2+ spark coupling in murine arterial smooth muscle cells
Cheng X, Jaggar J. Genetic ablation of caveolin-1 modifies Ca2+ spark coupling in murine arterial smooth muscle cells. AJP Heart And Circulatory Physiology 2006, 290: h2309-h2319. PMID: 16428350, PMCID: PMC1698957, DOI: 10.1152/ajpheart.01226.2005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBariumCadmiumCalcium SignalingCaveolin 1ElectrophysiologyEnzyme InhibitorsFluorescent DyesFura-2IndolesMiceMice, KnockoutMicroscopy, ConfocalMicroscopy, ElectronMuscle, Smooth, VascularMyocytes, Smooth MuscleNitroargininePatch-Clamp TechniquesRyanodine Receptor Calcium Release ChannelConceptsCerebral artery smooth muscle cellsSmooth muscle cellsArtery smooth muscle cellsMuscle cellsMurine arterial smooth muscle cellsGenetic ablationNitric oxide synthase activityVoltage-dependent calcium channelsArterial smooth muscle cellsOxide synthase activitySmooth muscle contractilityChannel blockersMuscle contractilityCalcium channelsCav-1-deficient cellsSpark regulationL-typeElevated intracellularSpark frequencyPotassium channelsSarcoplasmic reticulumCurrent activationCav-1Control cellsRelease channel
2005
Calcium release from ryanodine receptors in the nucleoplasmic reticulum
Marius P, Guerra MT, Nathanson MH, Ehrlich BE, Leite MF. Calcium release from ryanodine receptors in the nucleoplasmic reticulum. Cell Calcium 2005, 39: 65-73. PMID: 16289270, DOI: 10.1016/j.ceca.2005.09.010.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium ChannelsCalcium SignalingCell LineCell NucleusCytoplasmDantroleneEndoplasmic ReticulumInositol 1,4,5-TrisphosphateInositol 1,4,5-Trisphosphate ReceptorsMiceMicroscopy, FluorescenceMuscle, SkeletalNuclear EnvelopeReceptors, Cytoplasmic and NuclearRyanodine Receptor Calcium Release ChannelConceptsNucleoplasmic reticulumNuclear envelopeRyanodine receptorType 1 RyRGene transcriptionC2C12 cellsTrisphosphate receptorIntranuclear eventsCell typesReticulumDNA synthesisCell linesCell functionReticular networkSkeletal muscleRyR inhibitor dantroleneDiscrete regionsTwo-photon photoreleaseRyRsReceptorsNucleusCellsTranscriptionThe ryanodine receptor mediates early zymogen activation in pancreatitis
Husain SZ, Prasad P, Grant WM, Kolodecik TR, Nathanson MH, Gorelick FS. The ryanodine receptor mediates early zymogen activation in pancreatitis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 14386-14391. PMID: 16186498, PMCID: PMC1242288, DOI: 10.1073/pnas.0503215102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCeruletideDantroleneEnzyme PrecursorsMaleMicroscopy, ConfocalModels, BiologicalPancreas, ExocrinePancreatitisRatsRats, Sprague-DawleyRyanodine Receptor Calcium Release ChannelSecretory VesiclesTrypsinogen
2004
A “Synaptoplasmic Cistern” Mediates Rapid Inhibition of Cochlear Hair Cells
Lioudyno M, Hiel H, Kong JH, Katz E, Waldman E, Parameshwaran-Iyer S, Glowatzki E, Fuchs PA. A “Synaptoplasmic Cistern” Mediates Rapid Inhibition of Cochlear Hair Cells. Journal Of Neuroscience 2004, 24: 11160-11164. PMID: 15590932, PMCID: PMC6730265, DOI: 10.1523/jneurosci.3674-04.2004.Peer-Reviewed Original ResearchConceptsCalcium-induced calcium releaseCochlear hair cellsHair cellsCholinergic efferent neuronsOuter hair cellsExogenous AChEfferent neuronsSynaptic releaseSynaptic inputsRat cochleaApical turnAcetylcholine receptorsCalcium releaseCalcium entersPotassium channelsCation channelsSarcoplasmic reticulumAChType IReceptorsRapid inhibitionCisternsCellsNeuronsRelease
2002
Carbon Monoxide Dilates Cerebral Arterioles by Enhancing the Coupling of Ca2+ Sparks to Ca2+-Activated K+ Channels
Jaggar J, Leffler C, Cheranov S, Tcheranova D, E S, Cheng X. Carbon Monoxide Dilates Cerebral Arterioles by Enhancing the Coupling of Ca2+ Sparks to Ca2+-Activated K+ Channels. Circulation Research 2002, 91: 610-617. PMID: 12364389, DOI: 10.1161/01.res.0000036900.76780.95.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriolesCalcium Channel BlockersCalcium SignalingCarbon MonoxideCells, CulturedCerebral ArteriesCulture TechniquesElectric ConductivityHemeKineticsLysineMuscle, Smooth, VascularPotassium Channels, Calcium-ActivatedRyanodineRyanodine Receptor Calcium Release ChannelSignal TransductionSwineVasodilationVasodilator AgentsConceptsCerebral arteriolesArteriole smooth muscle cellsRelease channel blockerSmooth muscle cellsLarge-conductance Ca2Ryanodine-sensitive Ca2Enzyme heme oxygenasePial arteriolesCerebral circulationChannel blockersCoupling of Ca2Cellular signaling mechanismsPotent effectsArteriolesMuscle cellsHeme oxygenaseVasodilatorsPotent activatorControl conditionDilationPresent studySignaling mechanismCa2Source of controversyPercentage of Ca2
2001
Inhibition of Ryanodine Binding to Sarcoplasmic Reticulum Vesicles of Cardiac Muscle by Zn2+ Ions
Wang H, Wei Q, Cheng X, Chen K, Zhu P. Inhibition of Ryanodine Binding to Sarcoplasmic Reticulum Vesicles of Cardiac Muscle by Zn2+ Ions. Cellular Physiology And Biochemistry 2001, 11: 83-92. PMID: 11275686, DOI: 10.1159/000047795.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCattleDithiothreitolMyocardiumProtein IsoformsRyanodineRyanodine Receptor Calcium Release ChannelSarcoplasmic ReticulumZincConceptsInhibitory effectCardiac muscleModulators of RyRSarcoplasmic reticulumSkeletal muscleSarcoplasmic reticulum vesiclesMuscleScatchard analysisRyanodine receptorRyanodine bindingRyRsThiol-reducing agentsPhysiological significanceReticulum vesiclesHill coefficientObvious changeActivation siteMM dithiothreitol
2000
Chronic Unloading by Left Ventricular Assist Device Reverses Contractile Dysfunction and Alters Gene Expression in End-Stage Heart Failure
Heerdt P, Holmes J, Cai B, Barbone A, Madigan J, Reiken S, Lee D, Oz M, Marks A, Burkhoff D. Chronic Unloading by Left Ventricular Assist Device Reverses Contractile Dysfunction and Alters Gene Expression in End-Stage Heart Failure. Circulation 2000, 102: 2713-2719. PMID: 11094037, DOI: 10.1161/01.cir.102.22.2713.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBlotting, NorthernBlotting, WesternCalcium-Transporting ATPasesFemaleGene Expression RegulationHeart FailureHeart VentriclesHeart-Assist DevicesHumansMaleMiddle AgedMyocardial ContractionRNA, MessengerRyanodine Receptor Calcium Release ChannelSarcolemmaSodium-Hydrogen ExchangersConceptsEnd-stage heart failureLVAD supportHeart failureContractile strengthLVAD implantationSarcoplasmic reticular membranesNegative FFRIntact myocardiumVentricular assist deviceBlot analysisExpression of genesWestern blot analysisContractile dysfunctionSERCA2a proteinLV trabeculaeSubtype 2aChronic unloadingLV tissueAssist deviceContractile forcePatientsAlters gene expressionHigher mRNAReticular membranesRyanodine receptor
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