2025
A Honduran Prevalence Study on Soil-Transmitted Helminths Highlights Serological Antibodies to Tm-WAP49 as a Diagnostic Marker for Exposure to Human Trichuriasis.
Briggs N, Versteeg L, Mejia R, Pollet J, Villar M, Zhan B, Segal G, Novak S, Lenihan P, Musgrave P, Ellis V, Coello C, Sastry K, Craft J, Hotez P, Bottazzi M. A Honduran Prevalence Study on Soil-Transmitted Helminths Highlights Serological Antibodies to Tm-WAP49 as a Diagnostic Marker for Exposure to Human Trichuriasis. American Journal Of Tropical Medicine And Hygiene 2025 PMID: 39933187, DOI: 10.4269/ajtmh.24-0514.Peer-Reviewed Original ResearchSoil-transmitted helminthsReceiver-operating characteristic analysisSerological assaysFecal examinationDiagnostic toolIndirect ELISAGold standard testRoundworm infectionDiseases of humansRecombinant proteinsValidation cohortScreening cohortActive infectionInfections rankControl seraHuman trichuriasisDiagnostic markerRelationship to chronic diseasesClinical questionsWestern blottingDiagnostic potentialInfectionWhipwormCharacteristic analysisPrevalence studies
2024
Intrathecal lactate dehydrogenase A inhibitors FX11 and oxamate alleviate chronic constriction injury-induced nociceptive sensitization through neuroinflammation and angiogenesis
Cheng H, Chen N, Chen W, Wu Z, Sun Y, Teng W, Su F, Sung C, Wen Z. Intrathecal lactate dehydrogenase A inhibitors FX11 and oxamate alleviate chronic constriction injury-induced nociceptive sensitization through neuroinflammation and angiogenesis. The Journal Of Headache And Pain 2024, 25: 207. PMID: 39587478, PMCID: PMC11590346, DOI: 10.1186/s10194-024-01916-x.Peer-Reviewed Original ResearchConceptsLactate dehydrogenase ANeuropathic painNociceptive sensitizationLDHA inhibitorsTreat neuropathic painType of painUpregulation of glycolysisCCI-induced neuroinflammationCentral nervous systemBackgroundNeuropathic painNeuropathic ratsSpinal roleNociceptive behaviorBlood vessel growthImmunohistochemical analysisControl inflammationPainEffective treatmentConclusionIn conclusionHIF-1aCellular mechanismsNervous systemTherapeutic targetVessel growthWestern blottingThe Role of NOXA in Venetoclax Treatment Response in Multiple Myeloma
Shivaram S, Yan H, Tang H, Anderson H, Kaddoura M, Wiedmeier-Nutor J, Tian S, Howe M, Bolarinwa A, Zepeda Mendoza C, Gupta V, Lehman S, Mitsiades C, Bergsagel P, Braggio E, Boise L, Fonseca R, Maura F, Kumar S, Baughn L. The Role of NOXA in Venetoclax Treatment Response in Multiple Myeloma. Blood 2024, 144: 4635-4635. DOI: 10.1182/blood-2024-205385.Peer-Reviewed Original ResearchMayo Clinic cohortNoxa proteinMultiple myelomaG alleleIncreased expressionGene expressionCyclin D1Clinical cohortExpression of CCND1Cell linesAllele specific effectsBCL-2 inhibitor venetoclaxLevels of NoxaHistone mark H3K27acG risk allelePrimary cytogenetic abnormalityPlasma cell malignancyAssociated with increased expressionRNA sequencing dataHuman myeloma cell linesKO cell linesWestern blottingAnalyzed whole-exomeChIP-seqWhole genomeTargeting hypoxia and thrombospondin‐2 in diabetic wound healing
Huang Y, Xing H, Naud S, Kyriakides T. Targeting hypoxia and thrombospondin‐2 in diabetic wound healing. The FASEB Journal 2024, 38: e70091. PMID: 39383062, PMCID: PMC11486302, DOI: 10.1096/fj.202302429rrr.Peer-Reviewed Original ResearchConceptsThrombospondin-2Diabetic miceWound healingHIF-1aMatricellular protein thrombospondin-2Diabetic woundsImpaired wound healingWounds of diabetic miceDimethyloxalylglycine treatmentTargeting hypoxiaSustained hypoxiaDiabetic patientsTSP2 expressionCell dysfunctionIncreased neovascularizationDiabetic wound healingGenetic ablationDiabetic fibroblastsElevated glucoseReduced hypoxiaImprove healingImmunofluorescence analysisHIF-1a activationHypoxiaWestern blottingProtocol for isolating and identifying small extracellular vesicles derived from human umbilical cord mesenchymal stem cells
Chen Y, Qian H, Mak M, Tao Z. Protocol for isolating and identifying small extracellular vesicles derived from human umbilical cord mesenchymal stem cells. STAR Protocols 2024, 5: 103197. PMID: 39028618, PMCID: PMC11315167, DOI: 10.1016/j.xpro.2024.103197.Peer-Reviewed Original ResearchSmall extracellular vesiclesUmbilical cord mesenchymal stem cellsHuman umbilical cord mesenchymal stem cellsIsolation of small extracellular vesiclesLipid bilayer-enclosed particlesExtracellular vesiclesMesenchymal stem cellsNanoparticle tracking analysisAtomic force microscopeStem cellsMolecular markersLiving cellsTransmission electron microscopyWestern blottingIsolatesVesiclesCellsForce microscopeCEACAM5-Targeted Immuno-PET in Androgen Receptor–Negative Prostate Cancer
Imberti C, De Gregorio R, Korsen J, Hoang T, Khitrov S, Kalidindi T, Nandakumar S, Park J, Zaidi S, Pillarsetty N, Lewis J. CEACAM5-Targeted Immuno-PET in Androgen Receptor–Negative Prostate Cancer. Journal Of Nuclear Medicine 2024, 65: 1043-1050. PMID: 38782457, PMCID: PMC11218725, DOI: 10.2967/jnumed.123.267107.Peer-Reviewed Original ResearchConceptsNeuroendocrine prostate cancerCarcinoembryonic antigen-related cell adhesion molecule 5CEACAM5 expressionProstate cancerAggressive neuroendocrine prostate cancerAndrogen receptor (AR)-negativeEx vivo organ distributionXenograft prostate cancer modelsProstate cancer cell linesProstate cancer modelCell line LNCaP.Immuno-PET imagingCell line PC3Prostate cancer hallmarksCancer cell linesCancer modelsRadiolabeled antibodiesImmuno-PETSurface antigensProstatePET imagingCancerCancer hallmarksWestern blottingCell lines
2023
Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease
Bowen C, Lin Y, Zeng H, Nguyen H, Xiao H, Kumar P, Duong D, Espinosa‐Garcia C, Wulff H, Seyfried N, Rangaraju S. Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.078851.Peer-Reviewed Original ResearchKv1.3 blockadeKv1.3 channelsKv1.3 blockersMouse model of AD pathologyBlockade of Kv1.3Immune regulatory roleWestern blottingAlzheimer's diseaseCMV-Cre miceChannels in vitroPDZ-binding motifPlasma membrane proteinsInhibition in vivoCell type-specificPharmacological blockadeOlder 5XFAD miceCMV-CreFunction of microgliaChannel abundanceKv1.3Mouse modelBiotin ligaseFlow cytometryTrafficking proteinsBiotinylated proteinsCD19 Intron Retention Is Mechanism of CAR-T Treatment Resistance in Non-Hodgkin Lymphoma
Noble J, Locke F, Savid-Frontera C, Jain M, Turner J, Miranda J, Madanayake T, Naderinezhad S, Corallo S, Menges M, Reid K, Robinson T. CD19 Intron Retention Is Mechanism of CAR-T Treatment Resistance in Non-Hodgkin Lymphoma. Blood 2023, 142: 3506. DOI: 10.1182/blood-2023-185074.Peer-Reviewed Original ResearchNon-durable responseProgression free survivalSurface protein expressionB-cell acute lymphoblastic leukemiaCAR-TCAR-T treatmentFlow cytometryProtein expressionFree survivalCell linesWestern blottingRefractory diffuse large B-cell lymphomaMedian expressionChimeric antigen receptor T cellsJeKo-1Associated with progression free survivalDiffuse large B-cell lymphomaPre-treatment tumor biopsiesPre-treatment tumor samplesLarge B-cell lymphomaIntron 2CAR-T failureCD19 surface expressionR/R DLBCL patientsIntron 6High dietary K+ intake inhibits proximal tubule transport
Wang T, Liu T, Xu S, Frindt G, Weinstein A, Palmer L. High dietary K+ intake inhibits proximal tubule transport. American Journal Of Physiology. Renal Physiology 2023, 325: f224-f234. PMID: 37318989, PMCID: PMC10396284, DOI: 10.1152/ajprenal.00013.2023.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFree-flow micropunctureUrine volumeFractional excretion of Na<sup>+</sup>Proximal tubulesFiltration rateFractional NaNephron segmentsExcretion of Na<sup>+</sup>Dietary K<sup>+</sup>NHE3 protein expressionExchanger isoform 3Increased urine volumeIncreased fractional excretionVolume reabsorptionAbsolute NaKidney functionProtein expressionIsoform 3PT transportAnesthetized animalsReabsorptionWestern blottingRatsExcretionDesign of Class I/IV Bromodomain-Targeting Degraders for Chromatin Remodeling Complexes
Zahid H, Costello J, Li Y, Kimbrough J, Actis M, Rankovic Z, Yan Q, Pomerantz W. Design of Class I/IV Bromodomain-Targeting Degraders for Chromatin Remodeling Complexes. ACS Chemical Biology 2023, 18: 1278-1293. PMID: 37260298, PMCID: PMC10698694, DOI: 10.1021/acschembio.2c00902.Peer-Reviewed Original ResearchConceptsChromatin Remodeling ComplexNon-BET bromodomainsRemodeling complexProtein degradationHeterobifunctional moleculesBET familyProtein targetsPyrimidine base analogsNumber of degradersDegradersOncogenic roleTernary complexExit vectorsWestern blottingProteinFirst exampleClass IChallenging targetComplexesCECR2ChromatinBromodomainsBPTFFamilyNanoBRET
2022
MHC class II-restricted-presentationMHC class II and DM molecules in chickens: different yet again
Wise D, Halabi S, Afrache H, Fakiola M, Parker A, Kaufman J. MHC class II-restricted-presentationMHC class II and DM molecules in chickens: different yet again. Molecular Immunology 2022, 150: 32-33. DOI: 10.1016/j.molimm.2022.05.108.Peer-Reviewed Original ResearchIntestinal epithelial cellsClass II moleculesKO cellsClass II A genesII moleculesClass I systemResistance to infectious diseasesRT-PCRBF2 genesWestern blottingDM locusClass II BI systemA geneChicken MHCGenetic associationTapasin geneCRISPR-Cas9DMB1Tissue section stainingGenesBLB2Closely-linkedB2-microglobulinGut tissueCD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging.
Cui H, Xie N, Banerjee S, Dey T, Liu RM, Antony VB, Sanders YY, Adams TS, Gomez JL, Thannickal VJ, Kaminski N, Liu G. CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging. American Journal Of Respiratory And Critical Care Medicine 2022, 206: 459-475. PMID: 35687485, DOI: 10.1164/rccm.202109-2151oc.Peer-Reviewed Original ResearchConceptsIdiopathic pulmonary fibrosisLung fibrosisCD38 expressionAlveolar epithelial cell injuryEpithelial cell injuryEffective therapeutic strategyHuman lung parenchymaIPF lungsLung functionPulmonary fibrosisDisease progressionFibrotic lungsReal-time PCRYoung miceLung parenchymaOld miceCell injuryTherapeutic strategiesFibrosisPharmacological inactivationCD38Single-cell RNA sequencingFlow cytometryWestern blottingOld animals
2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENaKnockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1
Liu F, Peng S, Adelman RA, Rizzolo LJ. Knockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1. Investigative Ophthalmology & Visual Science 2021, 62: 14-14. PMID: 33591357, PMCID: PMC7900869, DOI: 10.1167/iovs.62.2.14.Peer-Reviewed Original ResearchConceptsSequestosome 1/p62Photoreceptor outer segmentsInternalized photoreceptor outer segmentsOxidative stress responseApical junctional complexActivation of AMPKTransepithelial electrical resistanceFluorescence-activated cell sortingWestern blottingClaudin-19Expression of proteinsSteady-state levelsProtein kinaseFilamentous actinEffect of AMPStress responseQuantitative RT-PCRKnockdownPOS degradationAMPKJunctional complexesEnzymatic activityCell sortingExpression of metallothioneinProteasome inhibitors
2020
The role of Hipk2-p53 pathways in arsenic-induced autistic behaviors: A translational study from rats to humans
Zhou H, Lin Y, Zhao W, Teng Y, Cui Y, Wang T, Li C, Jiang YH, Zhang JJ, Wang Y. The role of Hipk2-p53 pathways in arsenic-induced autistic behaviors: A translational study from rats to humans. Environmental Pollution 2020, 267: 115568. PMID: 33254717, DOI: 10.1016/j.envpol.2020.115568.Peer-Reviewed Original ResearchConceptsCase-control studySerum levelsArsenic exposureHealthy controlsNeuronal apoptosisFrontal cortexAge-matched healthy controlsGestational arsenic exposureHuman case-control studiesASD developmentHigher serum levelsAutistic behaviorASD childrenDose-dependent mannerAutism spectrum disorderPotential mediating pathwaysRat modelRat pupsKey molecular changesImmunohistochemistry analysisTranslational studiesWestern blottingMolecular changesTranslational strategiesMediating pathwaysAlleviation by Mahuang Fuzi and Shenzhuo Decoction in High Glucose‐Induced Podocyte Injury by Inhibiting the Activation of Wnt/β‐Catenin Signaling Pathway, Resulting in Activation of Podocyte Autophagy
Dai H, Liu F, Qiu X, Liu W, Dong Z, Jia Y, Feng Z, Liu Z, Zhao Q, Gao Y, Zhang Z, Gao C, Sun S, Tian X, Liu B. Alleviation by Mahuang Fuzi and Shenzhuo Decoction in High Glucose‐Induced Podocyte Injury by Inhibiting the Activation of Wnt/β‐Catenin Signaling Pathway, Resulting in Activation of Podocyte Autophagy. Evidence-based Complementary And Alternative Medicine 2020, 2020: 7809427. PMID: 32963573, PMCID: PMC7486640, DOI: 10.1155/2020/7809427.Peer-Reviewed Original ResearchDiabetic kidney diseaseHigh glucose-induced podocytesMahuang-FuziPodocyte injuryExcessive activationProtein expressionTreatment of DKDHigh glucoseTraditional Chinese medicine compoundWnt/β-Catenin Signaling PathwayInjury-related proteinsChinese medicine compoundΒ-Catenin Signaling PathwayPathway-related proteinsHigh glucose stimulationInhibition of autophagyKidney diseaseVariety of diseasesPodocyte autophagyFuzi decoctionOrgan fibrosisMetabolic diseasesGolden ChamberInhibitor DKK1Western blotting
2019
Reduction of AHI1 in the serum of Taiwanese with probable Alzheimer’s disease
Sheu JJ, Yang LY, Sanotra MR, Wang ST, Lu HT, Kam RSY, Hsu IU, Kao SH, Lee CK, Shieh JC, Lin YF. Reduction of AHI1 in the serum of Taiwanese with probable Alzheimer’s disease. Clinical Biochemistry 2019, 76: 24-30. PMID: 31786207, DOI: 10.1016/j.clinbiochem.2019.11.011.Peer-Reviewed Original ResearchConceptsAlzheimer's diseaseAD seraAD patientsEarly diagnosisAD model mouse brainsC-terminal APP fragmentsMini-Mental State Examination scoreSerum samplesHealthy control subjectsState Examination scoreBlood-based biomarkersModel mouse brainProbable Alzheimer's diseaseAge-dependent mannerGood diagnostic performanceHC seraControl subjectsAPP fragmentsAD biomarkersMouse brainYounger ageAD diagnosisNeuronal cellsWestern blottingDiseaseProgranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice
Wang C, Zhang L, Ndong J, Hettinghouse A, Sun G, Chen C, Zhang C, Liu R, Liu C. Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice. Journal Of Neuroinflammation 2019, 16: 238. PMID: 31775776, PMCID: PMC6882111, DOI: 10.1186/s12974-019-1630-1.Peer-Reviewed Original ResearchConceptsBasso Mouse ScaleNeurological recoverySpinal cord injuryCord injuryWestern blottingWild-type littermate miceMacrophages/microgliaPromising therapeutic approachWeight-drop techniqueNew therapeutic targetsHuge economic burdenPro-apoptotic effectsPLGA thermosensitive hydrogelFunctional recoveryPGRN deficiencyPGRN expressionLittermate miceIntrathecal spaceProgranulin deficiencyTherapeutic approachesTherapeutic targetEconomic burdenWT controlsDay 21Day 7Potential roles of aquaporin 9 in the pathogenesis of endometriosis
Choi YS, Park JH, Yoon JK, Yoon JS, Kim JS, Lee JH, Yun BH, Park JH, Seo SK, Cho S, Lee BS, Taylor HS. Potential roles of aquaporin 9 in the pathogenesis of endometriosis. Molecular Human Reproduction 2019, 25: 373-384. PMID: 31070762, DOI: 10.1093/molehr/gaz025.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsPathogenesis of endometriosisEndometrial stromal cellsPotential therapeutic targetExpression of MMP2P38 MAPK proteinWestern blot analysisControl patientsEndometrial expressionPhosphorylated ERK 1/2Ectopic endometriumEutopic endometriumInflammatory processLaparoscopic surgeryEndometriosisTherapeutic targetBenign conditionsAQP subtypesExpression of AQP9Stromal cellsTumor developmentWestern blottingAQP9 expressionHealing assaysERK 1/2Sustained‐release of hydrogen sulfide by GYY4137 protected diabetic‐induced kidney injury
Yu Y, Xu S, Ji D, Wang X, Wang T. Sustained‐release of hydrogen sulfide by GYY4137 protected diabetic‐induced kidney injury. The FASEB Journal 2019, 33: 567.2-567.2. DOI: 10.1096/fasebj.2019.33.1_supplement.567.2.Peer-Reviewed Original ResearchDKD miceDiabetic kidney diseaseWestern blottingLevels of apoptosisUntreated groupSustained-releaseUrine albuminTUNEL stainingSignaling moleculesPodocyte foot processesDiabetic kidney disease patientsAdministration of GYY4137Urinary albumin concentrationGaseous signaling moleculeProtective effectApoptosisCD-1 miceExpression of CBSBeta-lyaseExpression levelsFASEB JournalFoot processesHigh-fat dietFusion of foot processesMouse kidney
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